Main sol. 480c
Internal Solution-ID: S981
Equipment needed: Filter
Gassing station
Water bath
Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.33 | g | 0.329 | 6.157 | |
| CaCl2 x 2 H2O | 0.33 | g | 0.329 | 2.24 | |
| MgCl2 x 6 H2O | 0.33 | g | 0.329 | 1.62 | |
| KCl | 0.33 | g | 0.329 | 4.418 | |
| KH2PO4 | 0.33 | g | 0.329 | 2.42 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
MOPS buffer
(SIGMA M3183) |
3.90 | g | 3.892 | 18.6 | |
| Yeast extract | 0.10 | g | 0.1 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
|
Sulfur
(powder) |
10.00 | g | 9.98 | 311.195 | |
| Na-DL-lactate | 2.50 | g | 2.495 | 22.265 | |
| Na2CO3 | 1.00 | g | 0.998 | 9.416 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.50 | g | 0.499 | 2.078 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur, lactate, carbonate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 5.9 and sterilize medium by heating for 2- 3 hours in a boiling water bath on each of 3 successive days. Add lactate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary. | |||||
Media with this solution
| 480c | DESULFURELLA (K-119) MEDIUM |