# 1000: MJANHOX-NO3 MEDIUM WITH SUPPLEMENT


## Main sol. 1000

3	g	NaCl	
0.014	g	K2HPO4	
0.014	g	CaCl2 x 2 H2O	
0.04	g	NH4Cl	
0.34	g	MgSO4 x 7 H2O	
0.418	g	MgCl2 x 6 H2O	
0.033	g	KCl	
5	mg	Fe2(SO4)3 x n H2O	
5	µg	NiCl2 x 6 H2O	
5	µg	Na2SeO3 x 5 H2O	
0.5	g	Na2SiO3 x 9 H2O	
1	ml	**Trace mineral solution**	
1000	ml	Distilled water	

1. Before autoclaving, the pH of the Medium is adjusted with NaOH to 7.5-8.0. After autoclaving, the filter-sterilized vitamin solution (0.1% volume), and each of the separately autoclaved, concentrated solutions are added to the medium at a final concentration described below. Then a mix gas (80% H2, 20% CO2) is purged for 5 min. Finally, the mix gas is compressed into gas phase (> 80% volume of the tube or bottle) at 3 atm.

10	mM	Na2S2O3 x 5 H2O	
0.2	%	NaHCO3	
0.05	%	NaNO3	


## Trace mineral solution

1.5	g	Nitrilotriacetic acid	
0.5	g	MnSO4 x 2 H2O	
0.5	g	CoSO4 x 7 H2O	
0.18	g	ZnSO4 x 7 H2O	
0.01	g	CuSO4 x 5 H2O	
0.02	g	AlK(SO4)2 x 12 H2O	
0.01	g	H3BO3	
0.01	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water