# 1002: ECTOTHIORHODOSYNUS MEDIUM


## Main sol. 1002

0.5	g	KH2PO4	
0.5	g	NH4Cl	
20	g	NaCl	
0.2	g	MgCl2 x 6 H2O	
0.1	g	CaCl2	
5	g	Na2CO3	
5	g	NaHCO3	
1	g	Sodium acetate	
0.1	g	Yeast extract	
0.5	g	Na2S2O3 x 5 H2O	
0.1	g	Na2S x 9 H2O	
1	ml	**Trace element solution SL-6**	
20	µg	Vitamin B12	
1000	ml	Distilled water	

1. Prepare the NH4Cl, CaCl2, carbonate, bicarbonate and sulfide as a single sterile stock solution and add to the sterile medium after autoclaving. The pH should be adjust to 9-9.5.


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water