# 1006: ALLISONELLA MEDIUM


## Main sol. 1006

7.8	g	DL-Histidine	
4	g	Yeast extract	
1	g	Trypticase peptone (BD BBL)	
0.29	g	K2HPO4	
0.29	g	KH2PO4	
0.48	g	(NH4)2SO4	
0.48	g	NaCl	
0.1	g	MgSO4 x 7 H2O	
64	mg	CaCl2 x 2 H2O	
0.5	ml	Sodium resazurin (0.1% w/v)	
4	g	Na2CO3	
3.1	ml	**Volatile fatty acid mixture**	
0.5	g	L-Cysteine HCl x H2O	
1000	ml	Distilled water	

1. Dissolve ingredients (except carbonate, fatty acid mixture and cysteine), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate, fatty acid mixture and cysteine and adjust pH to 6.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.0, if necessary.


## Volatile fatty acid mixture

548.5	ml	Acetic acid	
193.5	ml	Propionic acid	
129	ml	Butyric acid	
32.25	ml	n-Valeric acid	
32.25	ml	iso-Butyric acid	
32.25	ml	DL-2-Methylbutyric acid	
32.25	ml	iso-Valeric acid