# 1014: THIOALKALIVIBRIO HALOPHILUS MEDIUM


## Main sol. 1014

175	g	NaCl	
1.5	g	K2HPO4	
0.5	g	NH4Cl	
1	ml	**Trace elements solution (Pfennig & Lippert,1966)**	
0.2	g	MgCl2 x 6 H2O	
0.25	g	MgSO4 x 7 H2O	
5	g	Na2S2O3	
4	g	NaHCO3	
1000	ml	Distilled water	

1. Dissolve sodium chloride, hydrogenphosphate, ammonium chloride and trace elements and fill solution in screw capped Erlenmeyer flasks to 10% of volume. Autoclave at 110°C for 30 min. Add magnesium chloride, magnesium sulfate, thiosulfate and bicarbonat from sterile stock solutions and adjust pH of the medium to 8.0 - 8.5.


## Trace elements solution (Pfennig & Lippert,1966)

5	g	EDTA	
2.2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.03	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.03	g	CuCl2 x 2 H2O	
0.03	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. pH 3.0-4.0