# 1019: ROUF'S MEDIUM


## Main sol. 1019

5	g	Yeast extract	
5	g	Peptone	
0.2	g	MgSO4 x 7 H2O	
0.05	g	CaCl2 x 6 H2O	
0.015	g	Fe(NH4)citrate	
0.05	g	MnSO4 x 4 H2O	
0.01	g	FeCl3 x 4 H2O	
10	ml	**Wolin's vitamin solution**	
1	ml	**Trace elements solution**	
1000	ml	Distilled water	
17	g	Agar, for solid medium	

1. pH  <pH:7.1> (adjusted with NaOH if needed)
2. The medium may be solifidied with 17 g/l agar.


## Trace elements solution

12.8	g	Nitrilotriacetic acid	
0.17	g	CoCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
0.1	g	FeSO4 x 7 H2O	
0.1	g	MnCl2 x 4 H2O	
0.1	g	NaCl	
0.1	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. First dissolve the nitrilotriacetic acid and adjust the pH to  <pH:6.5> with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH.


## Wolin's vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium D-(+)-pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	(DL)-alpha-Lipoic acid	
1000	ml	Distilled water