# 1019: ROUF'S MEDIUM


## Main sol. 1019

5	g	Yeast extract	
5	g	Peptone	
0.2	g	MgSO4 x 7 H2O	
0.05	g	CaCl2 x 6 H2O	
0.015	g	Fe(NH4)citrate	
0.05	g	MnSO4 x 4 H2O	
0.01	g	FeCl3 x 4 H2O	
10	ml	**Wolin's vitamin solution**	
1	ml	**Trace elements solution**	
1000	ml	Distilled water	
17	g	Agar, for solid medium	

1. pH 7.1 (adjusted with NaOH if needed)
2. The medium may be solifidied with 17 g/l agar.


## Trace elements solution

12.8	g	Nitrilotriacetic acid	
0.17	g	CoCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
0.1	g	FeSO4 x 7 H2O	
0.1	g	MnCl2 x 4 H2O	
0.1	g	NaCl	
0.1	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH.


## Wolin's vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium D-(+)-pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	(DL)-alpha-Lipoic acid	
1000	ml	Distilled water