# 1021: SODALIS GLOSSINIDIUS MEDIUM


## Main sol. 1021

900	ml	**Solution A**	
100	ml	**Solution B**	

1. Sterilize solution A and B separately at 121°C for 20 min. Cool to 50°C, then combine. To 4 parts of the above, add 1 part sterile foetal or new borne calf serum. Liquid medium should be filter sterilized instead of autoclaved.


## Solution A

6.2	g	Yeast extract	
8.1	g	Lactalbumin hydrolysate	
5	g	D-Glucose	
0.25	g	KCl	
0.12	g	MgCl2 x 6 H2O	
0.25	g	CaCl2 x 2 H2O	
8.7	g	NaCl	
0.15	g	NaHCO3	
17	g	Agar	
900	ml	Distilled water	

1. Adjust pH to 8.0.


## Solution B

0.28	g	NaH2PO4 x 2 H2O	
100	ml	Distilled water