# 1023: MANNING MEDIUM


## Main sol. 1023

6	g	(NH4)2SO4	
0.2	g	KCl	
0.2	g	K2HPO4	
1	g	MgSO4 x 7 H2O	
0.02	g	Ca(NO3)2	
1000	ml	Distilled water	

1. Solution A is separately autoclaved and added to the medium. The medium is supplemented with Solution B (yeast extract). Medium pH adjusted by 0.1 N H2SO4 to pH 2.5 - 2.7.


## Solution A

33.4	g	FeSO4 x 7 H2O	
1000	ml	Distilled water	


## Solution B

0.2	g	Yeast extract	
1000	ml	Distilled water