# 1025: MARICAULIS MEDIUM ## Main sol. 1025 30 g Sigma Sea salts 0.5 g NH4Cl 1000 ml Distilled water 20 ml **50xPYE** 5 ml Riboflavin (0.2 mg/ml) 2 ml Glucose (50 %) 1. Autoclave and cool the medium. Aseptically add from sterile stock solutions, 20 ml 50xPYE and 5 ml 0.2 mg/ml riboflavin (filter sterilised), and 2 ml 50% glucose per liter of medium. ## 50xPYE 100 g Peptone 50 g Yeast extract 1000 ml Distilled water