# 1038: ACIDICALDUS MEDIUM


## Main sol. 1038

0.45	g	(NH4)2SO4	
0.05	g	KCl	
0.5	g	MgSO4 x 7 H2O	
0.05	g	KH2PO4	
0.02	g	Ca(NO3)2 x 4 H2O	
1	g	D-Glucose	
0.2	g	Yeast extract (OXOID)	
1000	ml	Distilled water	

1. Dissolve ingredients except glucose and yeast extract, adjust pH to 2.5, dispense in suitable cultivation vessels and autoclave. Add glucose and yeast extract after autoclaving from sterile stock solutions.