# 1059: GEOALKALIBACTER MEDIUM


## Main sol. 1059

0.5	g	NH4Cl	
0.2	g	KCl	
0.1	g	MgCl2 x 6 H2O	
0.2	g	KH2PO4	
1	g	NaCl	
1	ml	**Trace element solution SL-10**	
1	ml	**Selenite-tungstate solution**	
3	g	Na2CO3	
10	g	NaHCO3	
10	g	Sulfur (powdered)	
0.1	g	Yeast extract	
2.5	g	Na-acetate	
1000	ml	Distilled water	

1. Dissolve ingredients (ecxept carbonate, hydrogencarbonate, sulfur, yeast extract and acetate), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate, adjust pH to 9.0 - 9.2, and dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur. Sterilize medium by autoclaving at <b>110°C</b> for 20 min. Add yeast extract and acetate from sterile anoxic stock solutions prepared under 100% N2 gas.


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.


## Selenite-tungstate solution

0.5	g	NaOH	
3	mg	Na2SeO3 x 5 H2O	
4	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water