# 1069: MODIFIED BIEBL AND PFENNIG'S MEDIUM


## Main sol. 1069

0.5	g	KH2PO4	
0.15	g	CaCl2 x 2 H2O	
2	g	MgSO4 x 7 H2O	
0.34	g	NH4Cl	
20	g	NaCl	
0.4	g	Yeast extract	
5	ml	Ferric citrate (0.1% w/v)	
1	ml	**Trace element solution SL-7**	
1000	ml	Distilled water	

1. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.


## Trace element solution SL-7

1000	ml	Distilled water	
1	ml	HCl (25%)	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
60	mg	H3BO3	
200	mg	CoCl2 x 6 H2O	
20	mg	CuCl2 x 2 H2O	
20	mg	NiCl2 x 6 H2O	
40	mg	Na2MoO4 x 2 H2O