# 1075: PPES-II AGAR MEDIUM


## Main sol. 1075

2	g	Peptone	
1	g	Proteose peptone no. 3 (Difco)	
1	g	Soytone	
1	g	Yeast extract	
0.1	g	Fe(III)-EDTA	
15	g	Agar	
1000	ml	**Artificial sea water**	

1. Adjust pH to 7.8.


## Artificial sea water

30	g	NaCl	
0.7	g	KCl	
10.8	g	MgCl2 x 6 H2O	
5.4	g	MgSO4 x 7 H2O	
1	g	CaCl2 x 2 H2O	
1000	ml	Distilled water