# 1077: PH MEDIUM


## Main sol. 1077

80	ml	**Solution 1**	
20	ml	**Solution 2**	

1. Combine solutions 1 and 2.


## Solution 1

1.68	g	PPLO broth	
80	ml	Distilled water	

1. Adjust pH to 6.5 and autoclave.


## Solution 2

18.7	ml	Horse serum (inactivated)	
0.936	ml	Yeast extract (25%, autoclaved)	
0.187	ml	Fish-Sperm DNA (filter-sterilized)	
0.187	ml	Distilled water	

1. Adjust pH to 7.8.