# 109: RHODOPSEUDOMONAS SULFOVIRIDIS MEDIUM ## Main sol. 109 0.3 g Yeast extract 1 g Disodium succinate 0.5 g Ammonium acetate 5 ml Fe(III) citrate (0.1% in H2O) 0.5 g KH2PO4 0.4 g MgSO4 x 7 H2O 0.4 g NaCl 0.4 g NH4Cl 0.05 g CaCl2 x 2 H2O 0.4 ml Vitamin B12 (10 mg in 100 ml H2O) 1 ml **Trace element solution SL-6** 0.3 g L-Cysteine HCl 0.5 ml Resazurin (0,1%) 1000 ml Distilled water 1. To medium add 0.1% sodium malate and 0.05% sodium thiosulfate (). Adjust pH to 6.8 and sterilize at 121°C for 15 min. The liquid medium is sterilized in rubber stoppered or screw-capped bottles under anaerobic conditions (Ref. 3365). After sterilization neutralized sterile sodium sulfide solution is added to a final concentration of 0.03% (see medium 27). 0.1 % Sodium malate 0.05 % Sodium Thiosulfate 2. Incubate in the light using a tungsten lamp. 3. Adjust pH to 6.8. ## Trace element solution SL-6 0.1 g ZnSO4 x 7 H2O 0.03 g MnCl2 x 4 H2O 0.3 g H3BO3 0.2 g CoCl2 x 6 H2O 0.01 g CuCl2 x 2 H2O 0.02 g NiCl2 x 6 H2O 0.03 g Na2MoO4 x 2 H2O 1000 ml Distilled water