# 1090: PYROBACULUM CALIDIFONTIS MEDIUM ## Main sol. 1090 10 g Tryptone (BD Bacto) 1 g Yeast extract 3 g Na2S2O3 x 5 H2O 1000 ml Distilled water 1. Dissolve ingredients and adjust to pH 7.0 with NaOH before autoclaving.