# 1091: HALOQUADRATUM WALSBYI MEDIUM


## Main sol. 1091

195	g	NaCl	
50	g	MgSO4 x 7 H2O	
35	g	MgCl2 x 6 H2O	
5	g	KCl	
0.25	g	NaHCO3	
1	g	NaNO3	
0.5	g	CaCl2 x 2 H2O	
0.05	g	KH2PO4	
0.03	g	NH4Cl	
20	ml	Tris buffer (1M)	
1000	ml	Distilled water	

1. Add after autoclaving: 

0.05	g	Yeast extract	
1	g	Sodium pyruvate	

2. Final pH 7.4
3. Yeast extract and sodium pyruvate are added from sterile (autoclaved) stock solutions to the respective concentrations.