# 113a: SULFURIMONAS HONGKONGENSIS MEDIUM


## Main sol. 113a

942	ml	**Solution A**	
40	ml	**Solution B**	
20	ml	**Solution C**	
1	ml	**Solution D**	
1	ml	**Solution E**	

1. Solutions A, B, and D are prepared under 100% N2 gas atmosphere and sterilized by autoclaving at 121⁰C for 15 min. Solution C is sterilized by filtration under an atmosphere of 80% N2 and 20% CO2 gas mixture. Solution E is prepared under a 100% N2 gas atmosphere and sterilized by filtration. Appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Check the pH of the complete medium and adjust to pH 7.0-7.2, if necessary.


## Solution A

25	g	NaCl	
2	g	KH2PO4	
2	g	KNO3	
1	g	NH4Cl	
0.8	g	MgSO4 x 7 H2O	
2	ml	**Trace element solution SL-4**	
940	ml	Distilled water	


## Solution B

5	g	Na2S2O3 x 5 H2O	
40	ml	Distilled water	


## Solution C

1	g	Na2CO3	
20	ml	Distilled water	


## Solution D

2	mg	FeSO4 x 7 H2O	
1	ml	H2SO4 (0.1 N)	


## Solution E

1	ml	**Wolin's vitamin solution (10x)**	


## Trace element solution SL-4

0.5	g	Na2-EDTA	
0.2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.


## Wolin's vitamin solution (10x)

20	mg	Biotin	
20	mg	Folic acid	
100	mg	Pyridoxine hydrochloride	
50	mg	Thiamine HCl	
50	mg	Riboflavin	
50	mg	Nicotinic acid	
50	mg	Calcium D-(+)-pantothenate	
1	mg	Vitamin B12	
50	mg	p-Aminobenzoic acid	
50	mg	(DL)-alpha-Lipoic acid	
1000	ml	Distilled water