# 1141: THERMOGYMNOMONAS MEDIUM ## Main sol. 1141 0.2 g (NH4)2SO4 3 g KH2PO4 0.5 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 1 g Yeast extract (Difco) 10 g Glucose 1000 ml Distilled water 1. Adjust pH to 3.0 with 10 N H2SO4. Separately autoclave yeast extract and glucose as 10% solutions, and add aseptically to the medium.