# 1146: VENENIVIBRIO STAGNISPUMANTIS MEDIUM


## Main sol. 1146

0.5	g	KCl	
1.36	g	MgCl2 x 6 H2O	
7	g	MgSO4 x 7 H2O	
2	g	Na2S2O3 x 5 H2O	
0.4	g	CaCl2 x 2 H2O	
0.2	g	NH4Cl	
0.25	g	KH2PO4	
10	ml	**Trace mineral solution**	
1.95	g	MES	
1000	ml	Distilled water	

1. Adjust the pH to 5.5 and gas the media with N2/CO2 (80/20) for 15 min at least. Fill 20 ml medium into 100 ml serum bottles, gas the flask containing the media with H2/CO2 (80/20) and seal with a rubber stopper. Autoclave the medium for 20 min at 121°C. After the inoculation the tubes are pressurized with H2/CO2/O2 (79%/15%/6%,170 kPa).


## Trace mineral solution

500	mg	Na-EDTA x 2 H2O	
150	mg	CoCl2 x 6 H2O	
100	mg	MnCl2 x 4 H2O	
100	mg	FeSO4 x 7 H2O	
100	mg	ZnCl2	
40	mg	AlCl3 x 6 H2O	
30	mg	Na-tungstate x 2 H2O	
20	mg	CuCl	
20	mg	NiSO4 x 6 H2O	
10	mg	Na2SeO3	
10	mg	H3BO3	
10	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. Adjust the pH of the solution to 3.0 with HCl.