# 1149: OCEANITHERMUS MEDIUM


## Main sol. 1149

0.78	g	K2HPO4	
0.75	g	KH2PO4	
10	ml	**Trace element solution**	
10	ml	**Vitamin solution**	
0.36	g	MgCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
0.53	g	NH4Cl	
0.14	g	Na2SO4	
20	g	NaCl	
2	g	Yeast extract	
1.7	g	NaNO3	
1000	ml	Distilled water	

1. Prepare the medium without vitamins, boil and cool under a nitrogen:carbon dioxide (80:20 v/v) atmosphere. Vitamins (filter sterilized and stored under nitrogen) and 100 ml 0.6 M NaHCO3 solution (sterilized and stored under nitrogen) are added from stock solutions.
2. The pH should be pH 6.5.


## Trace element solution

12.8	g	Nitrilotriacetic acid (NTA)	
1.35	g	FeCl3 x 6 H2O	
0.1	g	MnCl2 x 4 H2O	
0.024	g	CoCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
0.1	g	ZnCl2	
0.025	g	CuCl2 x 2 H2O	
0.01	g	H3BO3	
0.024	g	Na2MoO4 x 2 H2O	
1	g	NaCl	
0.12	g	NiCl2 x 6 H2O	
0.026	g	Na2SeO3 x 5 H2O	
1000	ml	Distilled water	


## Vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine-HCl x 2 H2O	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	D-Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water