# 1152: PM MEDIUM


## Main sol. 1152

4	g	KH2PO4	
11.2	g	Na2HPO4	
4	g	NaCl	
0.1	g	MgCl2 x 6 H2O	
0.1	g	MgSO4 x 7 H2O	
2	g	NH4Cl	
0.01	g	CaCl2	
x	µg	FeSO4 x 7 H2O	
10	ml	**Trace element solution SL-10**	
10	g	Na acetate	
1000	ml	Distilled water	
20	g	Agar, for solid medium	

1. Adjust to pH 7.2. The medium may be solidified by adding 20.0 g/l agar.


## Trace element solution SL-10

10	ml	HCl (25%; 7.7 M)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water