# 1154: GLUCOSE SULFIDE MEDIUM (Richard et al., 1985)


## Main sol. 1154

0.15	g	Glucose	
0.5	g	(NH4)2SO4	
0.1	g	CaCO3	
0.1	g	Ca(NO3)2	
0.05	g	KCl	
0.05	g	K2HPO4	
0.05	g	MgSO4 x 7 H2O	
0.187	g	Na2S x 9 H2O	
1	ml	**Vitamin solution**	
15	g	Agar, for solid medium	
1000	ml	Distilled water	

1. Adjust to pH 7.3. Sterilize by autoclaving at 121°C for 15 minutes.


## Vitamin solution

6	µg	Biotin	
0.1	µg	Calcium pantothenate	
0.1	µg	Co-carboxylase	
6	µg	Cyanocobalamin	
6	µg	Folic acid	
0.1	µg	Inositol	
0.1	µg	Niacin	
0.1	µg	p-Aminobenzoic acid	
0.1	µg	Pyridoxine	
0.1	µg	Riboflavine	
0.1	µg	Thiamine	
1000	ml	Distilled water	

1. filter-sterilized, added after autoclaving