# 1160: PAYNE, SEGHAL & GIBBONS MEDIUM


## Main sol. 1160

7.5	g	Casamino acids (Difco)	
10	g	Yeast extract	
3	g	Trisodium citrate	
2	g	KCl	
20	g	MgSO4 x 7 H2O	
36	mg	FeCl2 x 4 H2O	
0.36	mg	MnCl2 x 4 H2O	
250	g	NaCl	
1000	ml	Distilled water	
20	g	Agar, for solid medium	

1. Adjust pH to 7.4. For solid medium add 20.0 g agar.