# 1167: NSY-MEDIUM FOR POLYNUCLEOBACTER


## Main sol. 1167

1	g	Nutrient broth	
1	g	Soy peptone (e.g. Bacto Soytone)	
1	g	Yeast extract	
1000	ml	Tap water	

1. The following inorganic solution may substitute for tap water: 

75	mg	MgSO4 x 7 H2O	
43	mg	Ca(NO3)2 x 4 H2O	
16	mg	NaHCO3	
5	mg	KCl	
3.7	mg	K2HPO4 x 3 H2O	
1000	ml	Distilled water	
0.1	ml	**Trace element solution SL-4**	

2. Adjust to pH 7.2.


## Trace element solution SL-4

0.5	g	Na2-EDTA	
0.2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.