# 1171: METHYLOHALOMONAS MEDIUM


## Main sol. 1171

2	g	K2HPO4	
0.5	g	NH4Cl	
120	g	NaCl	
0.5	g	MgSO4 x 7 H2O	
0.024	g	NaS2O3 x 5 H2O	
1	ml	**Trace element solution SL-6**	
1.7	g	NaHCO3	
100	µg	Vitamin B12	
2	ml	Methanol	
1000	ml	Distilled water	

1. pH 7.5
2. Prepare the medium without methanol, vitamin B12 MgSO4 x 7 H2O, NaHCO3 and NaS2O3 x 5 H2O in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Add the methanol, MgSO4 x 7 H2O, NaHCO3 and NaS2O3 x 5 H2O from autoclaved stock solutions after sterilisation of the medium. Vitamin B12 is added from a filter sterilised stock solution to the sterile medium. Incubate the strains with shaking at 150 rpm. Growth is faster in the presence of 20mM sodium acetate instead of methanol.


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water