# 1176: HALOVIBRIO MEDIUM


## Main sol. 1176

120	g	NaCl	
3	g	K2HPO4	
0.5	g	NH4Cl	
1	ml	**Trace elements solution (Pfennig & Lippert,1966)**	
2.38	g	HEPES	
1000	ml	Distilled water	

1. After sterilization add from sterile stock solutions add: 

2	ml/l	MgSO4 x 7 H2O (1M)	
10	ml/l	Sodium acetate (2M)	
2	ml/l	Yeast extract (10%)	

2. Adjust pH to 7.5. Dispense the medium into sealed bottles and autoclave.


## Trace elements solution (Pfennig & Lippert,1966)

5	g	EDTA	
2.2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.03	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.03	g	CuCl2 x 2 H2O	
0.03	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. pH 3.0-4.0