# 118: DAP-NUTRIENT AGAR ## Main sol. 118 5 g Peptone 3 g Meat extract 100 mg Diaminopimelic acid 15 g Agar, if necessary 1000 ml Distilled water 1. Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.