# 1187: ANOYXNATRONUM MEDIUM


## Main sol. 1187

25	g	Na2CO3	
25	g	NaHCO3	
0.2	g	KCl	
0.1	g	MgCl2 x 6 H2O	
0.5	g	NH4Cl	
0.2	g	K2HPO4	
0.2	g	Yeast extract	
1	ml	**Trace element solution SL-10**	
1	ml	**Wolin's vitamin solution**	
0.7	g	Na2S x 9 H2O	
5	g	Glucose	
1000	ml	Distilled water	

1. pH 9.0
2. Prepare the medium anaerobically without the vitamins, Na2S, yeast extract NaHCO3, and Na2CO3. Boil the medium, cool under N2, add the NaHCO3, and Na2CO3, dispense under N2 and autoclave the medium. Add the vitamin solution, yeast extract, Na2S and glucose from sterile stock solution prepared under N2 to the cooled, autoclaved medium.


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.


## Wolin's vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium D-(+)-pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	(DL)-alpha-Lipoic acid	
1000	ml	Distilled water