# 1196: ZAVARZINELLA FORMOSA MEDIUM


## Main sol. 1196

0.1	g	KH2PO4	
0.1	g	MgSO4 x 7 H2O	
0.05	g	CaCl2 x 6 H2O	
0.01	g	NaCl	
1	g	N-Acetylglucosamine	
0.5	g	Glucose	
0.1	g	Peptone	
0.1	g	Yeast extract	
0.1	g	Casamino acids	
1	ml	**"Metals 44"**	
15	g	Agar, for solid medium	
1000	ml	Distilled water	

1. Final pH 5.8 - 6.0


## "Metals 44"

250	mg	Na-EDTA	
1095	mg	ZnSO4 x 7 H2O	
500	mg	FeSO4 x 7 H2O	
154	mg	MnSO4 x H2O	
39.2	mg	CuSO4 x 5 H2O	
24.8	mg	Co(NO3)2 x 6 H2O	
17.7	mg	Na2B4O7 x 10 H2O	
1000	ml	Distilled water	

1. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.