# 11a: MODIFIED MEDIUM 11


## Main sol. 11a

990	ml	**Main sol. 11**	
10	ml	**Vitamin solution**	

1. Prepare medium 11 with additional vitamins. Adjust pH to 6.2 - 6.5


## Main sol. 11

10	g	Casein peptone (tryptic digest)	
10	g	Meat extract	
5	g	Yeast extract	
20	g	Glucose	
1	g	Tween 80	
2	g	K2HPO4	
5	g	Na-acetate	
2	g	(NH4)3 citrate	
0.2	g	MgSO4 x 7 H2O	
0.05	g	MnSO4 x H2O	
1000	ml	Distilled water	

1. Adjust pH to 6.2 - 6.5.


## Vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine-HCl x 2 H2O	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
1	mg	p-Aminobenzoic acid	
0.01	mg	Vitamin B12	
1000	ml	Distilled water