# 1201: ALICYCLOBACILLUS FERROOXYDANS MEDIUM ## Main sol. 1201 930 ml **Solution A** 70 ml **Solution B** 1. Autoclave solution A, cool down to 50°C and combine solution A with 70 ml of solution B. ## Solution A 0.5 g MgSO4 x 7 H2O 0.4 g (NH4)2SO4 0.2 g K2HPO4 0.15 g K2S4O6 0.1 g KCl 0.01 g MnSO4 x H2O 0.2 g Yeast extract 930 ml Distilled water 1. Adjust pH to 1.8 - 2.5 with H2SO4. ## Solution B 20 g FeSO4 x 7 H2O 1. Dissolve in 0.2 N H2SO4 and fill up to a volume of 100 ml. Filter sterilize.