# 1203: FASTIDIOUS ANAEROBE AGAR


## Main sol. 1203

45.7	g	Fastidious Anaerobe Agar (acumedia)	
1000	ml	Deionized water	
10	%	Horse blood, adjust if required	

1. Allow to soak for 10 minutes, swirl to mix then sterilize by autoclaving at 121°C for 15 minutes. Cool to 47°C then aseptically add 5-10% of sterile defibrinated horse blood, mix well and pour plates. pH: 7.2 ± 0.2