# 1204: SPHAEROCHAETA MEDIUM


## Main sol. 1204

1	g	NaCl	
0.5	g	KCl	
0.3	g	NH4Cl	
0.2	g	KH2PO4	
0.15	g	Na2SO4	
0.4	g	MgCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
4	g	Yeast extract	
2	g	Casein peptone	
0.5	ml	Sodium resazurin (0.1% w/v)	
1	g	Na2CO3	
2	g	Maltose monohydrate	
0.8	g	DL-Dithiothreitol (DTT)	
1000	ml	Distilled water	

1. Dissolve ingredients except carbonate, maltose and DTT, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 minutes. Add maltose and DTT from sterile anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Check pH of complete medium and adjust to 7.4, if necessary.