# 121: MEDIUM FOR SULFOSPIRILLUM DSM 806


## Main sol. 121

10	g	Na-aspartate	
0.2	g	Yeast extract	
1	g	MgSO4 x 7 H2O	
28	mg	CaCl2 x 2 H2O	
0.75	g	K2HPO4	
0.25	g	NaH2PO4	
0.25	g	L-Cysteine HCl x H2O	
1	mg	Resazurin	
1	ml	**Trace element solution SL-10**	
1000	ml	Distilled water	

1. Adjust pH to 7.0. Phosphate and cysteine should be dissolved together in 1/10 vol. of water and sterilized separately.


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.