# 1218: ROSEINATRONOBACTER MONICUS MEDIUM


## Main sol. 1218

0.4	g	NH4Cl	
0.5	g	KH2PO4	
0.2	g	MgCl2 x 6 H2O	
0.5	g	Na2SO4	
0.4	g	NaNO3	
40	g	NaCl	
0.5	g	KCl	
10	g	NaHCO3	
5	g	Na2CO3	
1	g	Na2S2O3 x 5 H2O	
10	µg	Vitamin B12	
1	g	Sodium pyruvate	
1	g	Sodium acetate	
1	g	Peptone	
1	g	Yeast extract	
1	ml	**Trace element solution SL10**	
1000	ml	Distilled water	

1. Autoclave the NaHCO3 and Na2CO3 separately and add to the medium. Add the vitamin
B12 solution from a filter sterilised stock solution to the autoclaved, cooled medium. Final
is pH 9.0 - 9.5


## Trace element solution SL10

5	ml	HCl (concentrated)	
1.5	g	FeCl2 x 4 H2O	
0.07	g	ZnCl2	
0.2	g	MnCl2 x 4 H2O	
6	mg	H3BO3	
0.19	g	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
0.024	g	NiCl2 x 6 H2O	
0.036	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water