# 122: ACETIVIBRIO MEDIUM


## Main sol. 122

1.3	g	(NH4)2SO4	
2.6	g	MgCl2 x 6 H2O	
1.43	g	KH2PO4	
5.5	g	K2HPO4	
0.13	g	CaCl2 x 2 H2O	
6	g	Na2-ß-glycerophosphate x 5 H2O (MERCK 35675)	
1.1	ml	FeSO4 x 7 H2O (0.1% w/v in 0.1 N H2SO4)	
0.25	g	L-Glutathione (reduced)	
4.5	g	Yeast extract	
0.5	ml	Sodium resazurin (0.1% w/v)	
5	g	Cellobiose	
10	g	Cellulose (Avicel or MN 301) (optional)	
1000	ml	Distilled water	

1. Dissolve ingredients except cellobiose, sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Then adjust pH to 7.0 - 7.2, distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Cellobiose is added to the sterile medium from an anoxic 10% (w/v) stock solution prepared under 100% N2 gas and sterilized by filtration. Some strains can be adapted to cellulose as substrate using 10.00 g/l cellulose (Avicel or MN 301, MACHEREY-NAGEL).
2. Note: A white precipitate forms after mixing the ingredients of this medium, but this has no negative effect on growth.