# 1224: ECTOTHIORHODOSPIRA VARIABILIS MEDIUM


## Main sol. 1224

50	g	NaCl	
5	g	Na2CO3	
15	g	NaHCO3	
0.1	g	KCl	
0.5	g	K2HPO4	
0.2	g	MgCl2 x 6 H2O	
0.5	g	NH4Cl	
0.5	g	Na2S x 9 H2O	
0.5	g	Na2S2O3 x 5 H2O	
0.1	g	Yeast extract	
0.5	g	Na acetate	
1	ml	**Trace element solution SL-10**	
20	µg	Vitamin B12	
1000	ml	Distilled water	

1. Final pH 9.0 - 9.5
2. Prepare and boil the medium in the absence of Na2S x 9 H2O, NaHCO3, Vitamin B12, CaCl2 x 2 H2O, MgCl2 x 6 H2O. Cool under a stream of nitrogen and add the remaining components, except vitamin B12 and Na2S2O3 x 5 H2O. Dispense into tubes or bottle fitted with rubber stoppers and autoclave. Add the vitamin B12 (filter sterilised) and Na2S2O3 x 5 H2O from sterile stock solutions.


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.