# 123: MARINE AGAR


## Main sol. 123

1000	ml	**Synthetic sea water**	
5	g	Bacto peptone	
1	g	Yeast extract (Bacto)	
15	g	Agar	

1. To 1000 ml "Synthetic sea water" (see medium 79) add 5 g Bacto Peptone, 1 g Bacto Yeast extract, and 15 g agar. Alternatively medium 514 may be used.


## Synthetic sea water

24	g	NaCl	
11	g	MgCl2 x 6 H2O	
4	g	Na2SO4	
2	g	CaCl2 x 6 H2O	
0.7	g	KCl	
0.1	g	KBr	
0.03	g	H3BO3	
5	mg	NaSiO3 x 9 H2O	
0.04	g	SrCl2 x 6 H2O	
3	mg	NaF	
2	mg	NH4NO3	
1	mg	Fe(III)PO4 x 4 H2O	
1000	ml	Distilled water	

1. Adjust pH to 7.8.