# 1239: DESULFURISPIRILLUM MEDIUM


## Main sol. 1239

30	g	NaHCO3	
6	g	NaCl	
0.5	g	K2HPO4	
5.3	g	Na2CO3	
1	ml	**Trace element solution SL-12 B**	
1000	ml	Distilled water	
1	mM	MgSO4	
50	mg	Yeast extract	
20	mM	Sodium acetate	
20	mM	KNO3	
0.1	mM	Na2S	

1. Flush the medium with N2 and dispense 20 ml into 100ml serum bottles under an N2 atmosphere, seal with butyl rubber stoppers and autoclave. To the cooled. sterile medium add, from anaerobic, sterile stock solutions, MgSO4 (1 mM), yeast extract (50 mg/l), sodium acetate (20 mM), KNO3 (20 mM) and Na2S (0.1 mM).


## Trace element solution SL-12 B

3	g	Na2-EDTA	
1.1	g	FeSO4 x 7 H2O	
190	mg	CoCl2 x 6 H2O	
50	mg	MnCl2 x 2 H2O	
42	mg	ZnCl2	
24	mg	NiCl2 x 6 H2O	
18	mg	Na2MoO4 x 2 H2O	
300	mg	H3BO3	
2	mg	CuCl2 x 2 H2O	
1000	ml	Distilled water	

1. Adjust pH to 6.0.