# 1245: OTEB-MEDIUM


## Main sol. 1245

5	g	Proteose peptone no. 2	
5	g	Heart Infusion Broth	
5	g	Yeast extract	
2	g	Potassium Phosphate, dibasic	
5	g	Sodium Chloride	
0.1	g	Magnesium sulfate	
1.6	ml	Acetic acid	
0.6	ml	Propionic acid	
0.4	ml	n-Butyric acid	
0.1	ml	n-Valeric acid	
0.1	ml	Isovaleric acid	
0.1	ml	Isobutyric acid	
0.1	ml	2-Methylbutyric acid	
0.1	ml	Vitamin K1 (1% soln)	
0.5	g	L-Cysteine	
100	ml	Fetal bovine serum, inactivated	
100	ml	**Sugar Stock Solution**	
800	ml	Distilled water	

1. OTEB-medium is available from Anaerobe Systems (http://www.anaerobesystems.com)
2. The following text was taken from the above page: 

- ORAL TREPONEME ENRICHMENT BROTH (OTEB)

- Products: AS-603 Oral Treponeme Enrichment Broth (OTEB) 10 tubes / pkg
3. Intended Use: OTEB is an enriched medium for the isolation, cultivation and maintenance of Treponemes found as part of the oral flora.
4. OTEB is a complex peptone-yeast extract medium containing volatile fatty acids and serum for a source of long chain fatty acids. Volatile fatty acids and serum are necessary nutrients for recovery of Treponemes from the oral flora. Inoculation of the medium can be done using a gassing cannula or inside an anaerobic chamber. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.
5. Final pH 7.0 +/- 0.2 at 25 degrees C.
6. Final volume 7.0 ml +/- 0.7.


## Sugar Stock Solution

8	g	Glucose	
8	g	Fructose	
8	g	Sucrose	
8	g	Maltose	
8	g	Maltose	
8	g	Ribose	
8	g	Xylose	
8	g	Pectin	
8	g	Starch (soluble)	
8	g	Sodium pyruvate	
750	µg	Thiamine pyrophosphate	
1000	ml	Distilled water