# 1246: NSC3-MEDIUM WITH ACETATE


## Main sol. 1246

1	g	Na2S x 9 H2O	
0.25	g	NH4Cl	
0.25	g	K2HPO4	
1.5	g	NaHCO3	
10	g	NaCl	
0.44	g	Na-acetate	
1	ml	**Trace element solution SL-4**	
15	g	Agar	
999	ml	Distilled water	

1. Adjust pH to 8.5-9.0 before autoclaving. 


## Trace element solution SL-4

0.5	g	EDTA	
0.2	g	FeSO4 x 7 H2O	
100	ml	**Trace element solution SL-6**	
900	ml	Distilled water	


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water