# 1276: MEDIUM FOR CHELATOCOCCUS BUHARII


## Main sol. 1276

2.5	g	Yeast extract	
2.5	g	Tryptone	
1	g	Nitrilotriacetate (NTA)	
0.27	g	K2HPO4	
0.54	g	NaH2PO4 x 2 H2O	
2	g	MgSO4 x 7 H2O	
0.4	g	CaSO4 x 2 H2O	
0.5	g	Fe(III) citrate	
5	ml	**Trace element solution SL-6**	
18	g	Agar	
1000	ml	Distilled water	

1. Before adding agar, heat to boiling. After cooling adjust pH to pH 7.5. (pH drops strongly during heating).


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water