# 1324: MODIFIED METHYLOBACTERIUM MEDIUM


## Main sol. 1324

0.5	g	NH4Cl	
0.7	g	K2HPO4	
0.54	g	KH2PO4	
1	g	MgSO4 x 6 H2O	
0.2	g	CaCl2 x 2 H2O	
4	mg	FeSO4 x 7 H2O	
10	ml	**Trace element solution**	
1000	ml	Distilled water	
0.5	%	Methanol	
15	g	Agar, for solid medium	

1. Adjust pH to 6.8. Add sterile methanol to a concentration of 0.5% to the sterile medium. The medium may be solidified with 15 g/l agar


## Trace element solution

300	mg	H3BO3	
200	mg	CoCl2 x 6 H2O	
100	mg	ZnSO4 x 7 H2O	
30	mg	MnCl2 x 4 H2O	
10	mg	CuCl2 x 2 H2O	
20	mg	NiCl2 x 6 H2O	
60	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water