# 133: CARBON MONOXIDE OXIDIZER MEDIUM ## Main sol. 133 4.5 g Na2HPO4 x 12 H2O 0.75 g KH2PO4 1.5 g NH4Cl 0.2 g MgSO4 x 7 H2O 0.03 g CaCl2 x 2 H2O 18 mg Ferric ammonium citrate 1 ml **Trace element solution SL-6** 3 g Na-acetate (optional) 12 g Agar, for solid medium (optional) 1000 ml Distilled water 1. Dissolve ingredients, adjust pH to 7.0 and autoclave. 2. For chemoautotrophic growth incubate under a gas atmosphere of a) 20 - 80% carbon monoxide + 10% O2 + 70 - 10% N2 or b) 70% H2 + 20% O2 + 10% CO2 adding 2.50 g NaHCO3 per liter of medium. 3. For chemoorganotrophic growth add 3.00 g sodium acetate and incubate under air atmosphere. ## Trace element solution SL-6 0.1 g ZnSO4 x 7 H2O 0.03 g MnCl2 x 4 H2O 0.3 g H3BO3 0.2 g CoCl2 x 6 H2O 0.01 g CuCl2 x 2 H2O 0.02 g NiCl2 x 6 H2O 0.03 g Na2MoO4 x 2 H2O 1000 ml Distilled water