# 1330: ASAIA GYP MEDIUM


## Main sol. 1330

10	g	Glucose	
10	g	Glycerol	
10	g	Peptone	
5	g	Yeast extract	
7	g	CaCO3	
1000	ml	Distilled water	
15	g	Agar, for solid medium	

1. The medium may be solidified by adding 15 g/l agar. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. pH 6.8