# 1332: ALGORIPHAGUS AQUAEDUCTUS MEDIUM


## Main sol. 1332

20	g	Meat extract	
1	g	NH4Cl	
0.2	g	MgSO4 x 7 H2O	
0.01	g	CaCl2 x 2 H2O	
0.5	g	KH2PO4	
3	ml	**Seven vitamins solution**	
1	ml	**Trace element solution SL-8**	
996	ml	Distilled water	
15	g	Agar, for solid medium	

1. Adjust pH to 8.0 before autoclaving. The medium may be solidified by adding 15 g/l agar.


## Trace element solution SL-8

5.2	g	Na2-EDTA	
2	g	FeSO4 x 7 H2O	
190	mg	CoCl2 x 6 H2O	
100	mg	MnCl2 x 2 H2O	
150	mg	ZnSO4 x 7 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
62	mg	H3BO3	
17	mg	CuCl2 x 2 H2O	
1000	ml	Distilled water	

1. Adjust pH to 6.8.


## Seven vitamins solution

100	mg	Vitamin B12	
80	mg	p-Aminobenzoic acid	
20	mg	D-(+)-biotin	
200	mg	Nicotinic acid	
100	mg	Calcium pantothenate	
300	mg	Pyridoxine hydrochloride	
200	mg	Thiamine-HCl x 2 H2O	
1000	ml	Distilled water	

1. Filter sterilise and add to the medium after it has been autoclaved.