# 134: HALISCOMENOBACTER MEDIUM


## Main sol. 134

27	mg	KH2PO4	
40	mg	K2HPO4	
40	mg	Na2HPO4 x 2 H2O	
50	mg	CaCl2 x 2 H2O	
75	mg	MgSO4 x 7 H2O	
5	mg	FeCl3 x 6 H2O	
3	mg	MnSO4 x H2O	
1	ml	**Trace element solution SL-6**	
1.31	g	Glutamic acid	
2.5	mg	Trypticase soy broth (without glucose, BBL)	
0.4	mg	Thiamine-HCl x 2 H2O	
0.01	mg	Vitamin B12	
2	g	Glucose	
1000	ml	Distilled water	

1. Adjust pH to 7.5. The vitamins are sterilized by filtration. The glucose is dissolved in 5 ml of distilled water and sterilized separately by autoclaving.


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water