# 1352: FUCHSIELLA ALKALIACETIGENA MEDIUM


## Main sol. 1352

0.2	g	KH2PO4	
0.1	g	MgCl2 x 6 H2O	
0.5	g	NH4Cl	
0.2	g	KCl	
60	g	NaCl	
68.3	g	Na2CO3	
38.3	g	NaHCO3	
1	ml	**Trace element solution SL-4**	
0.5	mg	Resazurin	
10	ml	**Wolin's vitamin solution**	
0.05	g	Yeast extract	
10	ml	Ethanol (50% v/v)	
1	g	Na2S x 9 H2O	
0.85	g	NaNO3	
1000	ml	Distilled water	

1. pH 8.8- 9.5; gas atmosphere: 100% N2.
2. Dissolve ingredients except the sodium carbonate, sodium bicarbonate, ethanol, and sodium sulphide. Boil medium for a few minutes and cool to room temperature while flushing with N2. Add and dissolve sodium carbonate, sodium bicarbonate and sodium sulphide while gassing the head space only. Dispense and autoclave under N2. Before use add 10 ml/l (v/v) of 50% (v/v) ethanol solution (flushed and autoclaved under N2).


## Trace element solution SL-4

0.5	g	EDTA	
0.2	g	FeSO4 x 7 H2O	
100	ml	**Trace element solution SL-6**	
900	ml	Distilled water	


## Wolin's vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium D-(+)-pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	(DL)-alpha-Lipoic acid	
1000	ml	Distilled water	


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water