# 1353: YTBC - MEDIUM


## Main sol. 1353

1	g	Yeast extract	
5	g	Tryptone	
5	g	Casamino acids	
5	g	Beef extract (Lab-Lemco-Powder)	
1	g	K2HPO4	
1	g	NH4NO3	
0.25	g	Na2S x 9 H2O	
0.25	g	Cysteine HCl	
7.5	mg	NiCl2 x 6 H2O	
5	ml	**Trace element solution**	
0.5	ml	Resazurin (0.1% v/v)	
250	ml	**Artifical seawater solution**	
750	ml	Distilled water	

1. Prepare the liquid medium without Na2S and cysteine-HCl. Boil the medium in a water bath and cool under N2:CO2 (80:20 v/v) to room temperature. Adjust to pH 7.0 with 10 M NaOH solution. Dispense the medium into Hungate tubes or serum bottles. Sterilize by autoclaving. Before inoculatiing add Na2S and cysteine-HCl from sterile stock solutions.


## Trace element solution

1	g	FePO4	
0.01	g	Na2WO4 x 2 H2O	
0.01	g	Na2SO3 x 5 H2O	
500	ml	Distilled water	


## Artifical seawater solution

20	g	NaCl	
3	g	MgCl2 x 6 H2O	
6	g	MgSO4 x 7 H2O	
0.2	g	NaHCO3	
0.3	g	CaCl2 x 2 H2O	
0.5	g	KCl	
0.42	g	KH2PO4	
0.05	g	NaBr	
0.02	g	SrCl2	
0.01	g	Fe(NH4)citrate	
1	g	(NH4)2SO4	
1000	ml	Distilled water